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食品研究与开发:2017,38(10):110-112+172
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利用荧光定量PCR技术鉴别畜禽肉中猪源性成分
陆俊贤,唐修君,樊艳凤,贾晓旭,葛庆联,顾荣,王珏,高玉时*
(中国农业科学院家禽研究所,农业部家禽品质监督检验测试中心,江苏 扬州 225125)
The Identification of Pig Origin Ingredients in Livestock and Poultry Meat Based on Fluorogenic Quantitative PCR
LU Jun-xian, TANG Xiu-jun, FAN Yan-feng, JIA Xiao-xu, GE Qing-lian, GU Rong, WANG Jue, GAO Yu-shi*
(Institute of Poultry, Chinese Academy of Agricultural Sciences, Quality Inspection Center for Poultry Products, Ministry of Agriculture, Yangzhou 225125, Jiangsu, China)
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投稿时间:2016-08-24    
中文摘要: 以猪特异性基因序列为靶位点设计特异性引物,以常见畜禽肉包括猪肉、羊肉、兔肉、牛肉、鸽肉、鹌鹑肉、鸡 肉、鸭肉、鹅肉等参考动物肌肉 DNA 为模板,进行荧光定量 PCR 扩增,建立猪源性成分荧光定量 PCR 检测方法;并将猪 肉 DNA 模板浓度进行 8 个梯度稀释,检测其灵敏度。结果显示,该方法能够有效对猪源性成分进行快速检测,具有较强 的特异性,灵敏度较高,可快速准确地鉴别畜禽肉中猪源性成分。
Abstract:In order to establish a fluorogenic quantitative PCR method, which could identify the components of pig origin, specific genes sequences of pig was used as target sites, and the specific primers were designed. The DNA of common livestock and poultry meat including mutton, pork, rabbit meat, beef, pigeon meat, quail meat, chicken, duck and goose were used as template. And the template was diluted in eight gradients, for de- tecting the sensitivity. The results showed that the established method could detect the pig origin effectively, which had a quite specificity and high sensitivity. The method could detect the pig origin in livestock and poultry meat quickly and accurately.
文章编号:201710026     中图分类号:    文献标志码:A
基金项目:扬州市社会发展前瞻性研究项目(YZ2014188);扬州市科 技公共服务平台建设(YZ2015162);2016 国家农产品质量安全风险 评估项目(GJFP2016007)
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