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投稿时间：2016-08-10
投稿时间：2016-08-10
中文摘要: 对费氏丙酸杆菌（Propionibacterium freudenreichii CS1420）发酵培养基的碳源进行优化，旨在提高菌体密度。结 果表明，以乳酸钠作为单一碳源时，当乳酸钠添加量为 32 g/L 时，菌体生长量最大，OD600nm 为 1.95；以葡萄糖作为单一碳 源时，当葡萄糖添加量为 8 g/L 时，菌体生长量最大，OD600nm 为 1.242；以乳酸钠和葡萄糖作为复合碳源时，当碳源添加量 为 18.8g/L 乳酸钠+1.2g/L 葡萄糖时，菌体生长量最大，OD600nm为 2.06，这说明，复合碳源可以有效的提高菌体密度。
Abstract:The effects of the carbon sources of the medium on the growth of Propionibacterium freudenreichii CS1420 were studied in order to increase the cell density. The results showed： the growth rate of the cell was the largest when sodium lactate was used as a single carbon source and the amount of sodium lactate was 32g/L， OD600nm was 1.95. The growth rate of the cell was the largest when glucose was used as a single carbon source and the amount of sodium lactate was 8 g/L， OD600nm was 1.242. The growth rate of the cell was the largest when glu－ cose and sodium lactate were used as compounded carbons and the amount of sodium lactate was 18.8 g/L sodi－ um lactate， 1.2 g/L glucose， OD600nm was 2.06. This showed that the composite carbon source can effectively im－ prove the cell density.
文章编号：201709039 中图分类号： 文献标志码：A
基金项目:江苏省大学生实践创新训练项目（201610333036Y）；常熟 理工学院大学生实践创新训练项目
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