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投稿时间：2016-08-13
投稿时间：2016-08-13
中文摘要: 以豆渣为培养基，利用羊肚菌进行半固体发酵，通过正交试验优化发酵条件，得到最佳的发酵条件为料液比 1 ∶ 9（g/mL），接种量 3.0 %，发酵时间 9 d，发酵产物中多糖（CMP，Crude Morchella Polysaccharide）含量达到 8.86 %。同时，对 CMP 的功能性进行评价，结合胆酸盐能力试验表明，CMP 对 3 种胆酸盐（无水胆酸盐、脱氧胆酸盐、牛磺胆酸盐）均有结 合效果，其中对脱氧胆酸钠的结合量最高，为（0.16±0.02）mmol/L，证明 CMP 有降血脂功能。体外抗氧化试验表明，CMP 在浓度为 10 mg/mL 时对羟自由基的清除能力达 98.9 %，显示其具有良好的抗氧化性。
Abstract:The semisolid fermentation by Morchella esculenta using soybean curd residue was studied. Orthogo－ nal experimental design was employed to optimize the fermentation conditions. The optimal fermentation condi tions achieved for crude polysaccharide production 8.86 % were feed liquid ratio 1 ∶ 9 （g/mL）， inoculum size 3.0 % and fermentation time 9 days，respectively. The crude polysaccharide （CMP） was extracted from the fer－ mentation substrate，then the function of CMP was evaluated. The results on the ability of bile salt -binding showed that CMP had binding capacities for three kinds of cholates （anhydrous cholate， deoxycholate， tauro－ cholate），which demonstrated that CMP had hypolipidemic effect. Meanwhile， CMP exhibited a positive antioxi－ dant activity.
文章编号：201709033 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| YAO Heng， ZHU Hui， CHEN Ye， LI Shu-hong* | College of Food Engineering and Biotechnology， Tianjin University of Science & Technology， Tianjin 300457， China |
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