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食品研究与开发:2017,38(9):65-69
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响应面法优化金柚核中类柠檬苦素的 提取工艺
张声源1,邹浩元1,杨宇辉1,周铭1,陈一村2,罗铝铿1,李春泉3,聂华1,*
(1. 嘉应学院 医学院,广东 梅州 514031;2. 汕头大学 医学院,广东 汕头 515041; 3. 广东汇群中药饮片股份有限公司,广东 汕头 515041)
Optimization of Extraction Process for Limonins from Gold Pomelo Seeds by Response Surface Methodology
ZHANG Sheng-Yuan1, ZOU Hao-yuan1, YANG Yu-hui1, ZHOU Ming1, CHEN Yi-cun2, LUO Lü-keng1, LI Chun-quan3, NIE Hua1,*
(1. Medical College, Jiaying University, Meizhou 514031, Guangdong, China; 2. Medical College, Shantou University, Shantou 515041, Guangdong, China; 3. Traditional Chinese Medicine Company of Huiqun,Shantou 515041, Guangdong, China)
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投稿时间:2016-08-03    
中文摘要: 在单因素试验的基础上,选择超声时间、超声温度和料液比为自变量,柚核中柠檬苦素类物质的提取率为响应 值,进行 Box-Benhnken 中心组合试验设计,运用响应面分析法优化梅州金柚核柠檬苦素类物质的提取工艺。结果表明, 梅州金柚核柠檬苦素类物质超声提取最佳工艺为提取温度 50 ℃,料液比 1 ∶ 20(g/mL),超声时间 20 min。此工艺下柠檬 苦素类物质提取率为 0.491 mg/g(RSD=1.13 %,n=3),与理论值 0.487 mg/g 偏差很小。
Abstract:Based on the experiment of single factor,ultrasonic time,ultrasonic temperature,and solid -liquid ratio were selected to design Box-Behnken central composite. The optimum extraction conditions confirmed by the response surface methodology. Optimum extraction conditions were as follows:extraction time of 20 min, solid/liquid ratio of 1 ∶ 20(g/mL) and temperature of 50 ℃. Under these conditions,yield of total limonins was 0.491 (mg/g)(RSD=1.13 %, n =3),whose deviation was small by comparing with the theoretical value of 0.487(mg/g).
文章编号:201709015     中图分类号:    文献标志码:A
基金项目:广东省科技计划项目(2014A020221061, 2015A090905019); 广东省医学科研基金项目(A2015629,A2015354);梅州市产业技术 研究与开发资金计划项目(201411)
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