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投稿时间：2016-06-08
投稿时间：2016-06-08
中文摘要: 采用水提醇沉法提取裂褶菌多糖，用磺酰化法对其进行硫酸酯化，经红外光谱定性分析，硫酸钡比浊法定量计 算得出硫酸基的含量。采用邻二氮菲-Fe2+氧化法测定裂褶菌多糖和裂褶菌硫酸酯对羟自由基的清除作用。结果表明：硫 酸基含量为 13.9 %，硫酸基取代度（DS）为 1.26 时，硫酸酯化裂褶菌多糖的抗氧化活性比裂褶菌多糖增加 2.6 倍。硫酸酯 化修饰能提高裂褶菌多糖的抗氧化活性。
Abstract:The Schizophyllan polysaccharide was extracted by water extraction and ethanol precipitation method. The polysaccharide esterification were synthetized by chlorosulfonic acid method and was confirmed by infrared spectroscopy. The content of sulfate radical was determined by barium sulfate turbidity. The effects of polysaccharides scavenging hydroxyl were determined by phenanthroline-metal iron-H2O2 system. The results showed that： when sulfate replaced was 13.9 %， sulfation rate of 1.26 sulfated Schizophyllan polysaccharide an－ tioxidant activity increased by 2.6 times than the unmodified polysaccharide. The antioxidant activity of Schizo－ phyllan polysaccharide can be improved through sulfation．
文章编号：201708004 中图分类号： 文献标志码：A
基金项目:河南省科技攻关项目（142102210222c）
| Author Name | Affiliation |
| ZHANG Sui-shuan， CAI Hua-zhen | Henan Vocational College of Quality Engineering， Pingdingshan 467000， Henan， China |
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