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食品研究与开发:2017,38(7):102-105
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超临界CO2萃取麦冬挥发油的GC-MS分析
吴洪伟,吴岳滨,吴观健,黄颖颀,王兆玉*
(广东药科大学 生命科学与生物制药学院 广东省生物技术候选药物研究重点实验室, 广东 广州 510006)
Analysis of the Chemical Components of Essential Oil Extracted by Supercritical CO2 Fluid and Steam Distillation from Ophiopogon japonicus(L. f.) Ker-Gawl
WU Hong-wei, WU Yue-bin, WU Guan-jian, HUANG Ying-qi, WANG Zhao-yu*
(Guangdong Provincial Key Laboratory of Biotechnology Candidate Drug Research, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China)
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投稿时间:2016-06-17    
中文摘要: 采用超临界 CO2 萃取法(SFE-CO2)提取麦冬挥发油,进而采用气相色谱-质谱联用技术分析其化学成分。采用 SFE-CO2 法提取麦冬块根中的挥发油,用气相色谱-质谱联用仪(GC-MS)分析鉴定挥发油的化学成分,用面积归一化法 测定各化合物的相对含量。通过质谱库检索鉴定出 69 种化合物成分,占挥发油总含量的 97.12 %。研究成果可用作现代 化中药制剂生产、麦冬药材品质鉴定的参考。
Abstract:To analyse the chemical components of essential oil prepared by supercritical CO2 fluid extraction (SFE-CO2) from Ophiopogon japonicus (L. f.) Ker-Gawl root tuber. The essential oil of O. japonicus were ex- tracted by SFE-CO2. The chemical components of essential oil were separated and analyzed by gas chromatog- raphy-mass spectrometry (GC-MS). Their relative contents were determined by normalization of peak area. 69 compounds were detected in the essential oil, which accounted for 97.12 % of the total content of essential oil. The results can be used in the modern traditional Chinese medicine industry or quality identification of O. japonicus materials.
文章编号:201707022     中图分类号:10.3969/j.issn.1005-6521.2017.07.022    文献标志码:A
基金项目:国家自然科学基金(31201236);2015 年国家级大学生创 新创业训练计划项目(51307166);贵州省科技厅、遵义市政府、赤水 市政府 2015 年省市县三方科技合作金钗石斛产品研发与产业化项 目(赤科省市合[2016]2,赤科省市合[2016]7)
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