Abstract:Using the macroporous adsorptive resins， the purification conditions for total phenolic content（TPC） from the flower of Paeonia lactiflora Palla and the antioxidant capacity of the TPC were studied after purification. Static and dynamic experimental methods were performed to determine the purification conditions， and the con－ tents of the purified TPC were calculated. Among the 9 macroporous resins tested， HPD100 resin was the best in adsorption effect and desorption effect. At room temperature， when 50 % of ethanol was used as desorption solu－ tion， adsorption and desorption flow velocities were both 10 mL/min， the content of the TPC purified from the flower of Paeonia lactiflora Palla was 721.72 mg GAE/g dry weight， and it was 1.57 times higher compared with that before purification. The antioxidant activities of purified extracts measured by 2，2-diphenyl-1-picrylhy－ drazyl radical scavenging activity assay（DPPH）， ferric reducing antioxidant power assay（FRAP）， and ABTS+ radical scavenging activity （ABTS）assay were 1.35， 1.44 ，1.62 times higher than those of crude extracts before purification， respectively.