本文已被：浏览 1519次 下载 652次
投稿时间：2016-07-11
投稿时间：2016-07-11
中文摘要: 玉米赤霉醇是一种违法的畜禽增重剂，国家明确将其列入非食用物质。针对牛奶样品前处理方法进行优化，采 用高效液相色谱-三重四级杆串联质谱联用仪（HPLC-MS/MS）法测定牛奶中两种玉米赤霉醇的含量。样品直接采用乙 腈提取，Waters MAX 固相萃取小柱净化，负离子模式下，以乙腈-水为流动相进行梯度洗脱，以多反应监测（MRM）模式 记录玉米赤霉醇的离子碎片响应进行定性定量分析。结果表明：采用优化后的提取方法、色谱条件及质谱条件，两种玉 米赤霉醇的线性关系良好，线性范围为 2.5 ng/mL~80.0 ng/mL，线性相关系数 R2>0.999，α、β-玉米赤霉醇的平均回收率分 别为 108.5 %和 102.1 %。该法简单、快捷，确证可靠，适用于牛奶基质样品中玉米赤霉醇类物质的检测。
中文关键词: 牛奶 玉米赤霉醇 高效液相色谱-三重四级杆串联质谱 方法优化
Abstract:Zeranol is one of the extender for livestock. It is included in the non edible substance list. A method for determination of zeranol in milk by HPLC-MS/MS was described. The samples were extracted with acetoni－ trile， cleaned up with Waters MAX-SPE. After elution， the samples were analysed by MRM method in Negative mode. Acetonitrile-water was used as mobile phase and Ion fragment response was used to quantitative analysis. The linear range was 2.5 ng/mL-80.0 ng/mL， R2>0.999. Recoveries were 108.5 % and 102.1 %. The method is simple， rapid and reliable. It can be speculated that this method would be applied in the field of zeranol detec－ tion in milk matrix.
keywords: milk zeranol HPLC-MS/MS method optimization
文章编号：201706036 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| ZHU Jie， MA Gui-juan， MA Xue-mei | Ningxia Hui Autonomous Region Food Testing Center， Ningxia 750004， Yinchuan， China |
引用文本：
津ICP备20000603号-1