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投稿时间：2016-07-05
投稿时间：2016-07-05
中文摘要: 根据槲皮素能与铝离子形成稳定的荧光络合物，建立同步荧光法测定荷叶中总黄酮含量的方法。以槲皮素为 标样，选择发射波长与激发波长的波长差△λ 为 40 nm 扫描同步荧光光谱，测定荷叶中总黄酮含量，并进行加标回收试 验验证方法的可靠性。试验结果表明，槲皮素浓度在 0.008 μg/mL~0.40 μg/mL 范围内与荧光强度具有良好的线性关系， 线性回归方程： y=5 780x+3.27，相关系数 R 为 0.999 0。
Abstract:On the basis of reaction of quercetin with Alumimum ion to form fluorescent material， a new method of determining total flavonoids in lotus leaves was established. The content of total flavonoids in lotus leaves was determined， taking quercetin as standard sample， selecting △λ=40 nm and scanning by synchronous fluorom－ etry. Then the accuracy was inspected by recovery test. The experimental results showed that when the quercetin concentration ranged from 0.008 μg/mL to 0.40 μg/mL， fluorescence intensity and quercetin concentration showed the good linear relationship，the linearity relationship was y=5 780x+3.27， the correlation coefficient R was 0.999 0.
文章编号：201706033 中图分类号： 文献标志码：A
基金项目:江苏经贸职业技术学院科研基金资助项目（JSJM15023）
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