Abstract:The purpose of the present study was to investigate the toxicity of maple sugar on rats. Acute Toxicity test： Used limited method， ICR mice after a fast of 16 h， can not help but water， observed in 14 days after giv－ en subjects by oral in 15.0 g/kg BW dose . Ames test： Set up 5 000， 1 000， 200， 40， 8 μg/dish 5 dose groups， untreated group， solvent group， positive control group. Used plate incorporation method， counted the number of reverse colonies with 5 kinds of histidine nutrition deficiency Strain TA97，TA98，TA100，TA102 and TA1535. Mammalian Erythocyte Micronucleus test： Seted 10.0， 5.0， 2.5 g/kg BW three dose groups， cyclophosphamide group and negative control group. Two oral administration during 24 h.calculated proportion of bone marrow polychromatic erythrocytes in total red blood cells. 90 day feeding test： Seted 8.0， 6.0 4.0 g/kg BW three dose groups. Mixed feed for 90 days， blank control group was given normal feed. Observed body weight， food utiliza－ tion， blood routine， blood biochemical indexes and pathological examination. The result of Ames text and Mammalian Erythocyte Micronucleus test of maple sugar was negative. There was no significant difference com－ pared to blank control group about various index of 90 days feeding text. Maple sugar is confirmed edible safety by system tests in vivo and in vitro test at a certain dose range.