Abstract:This paper studied the influence of comfrey extract on alleviating sport fatigue of athletes after long distance running training of 1 month. The results showed that： the average heart rate and max heart rate of ob－ servation group after training appeared significantly enhanced than control groups （P<0.05）， and the morning heart rate of observation group significantly decline than the control groups （P<0.05）， The sleep quality，diet condition and body weight were significantly higher than the control group （P<0.05）， The average exercise power， maximal anaerobic power and maximum aerobic power of observation group were significantly higher than the control group（P<0.05）； the creatine kinase and blood urea nitrogen of observation group were signifi－ cantly higher than the control group （P<0.05）， and hemoglobin and testosterone of observation group were sig－ nificantly decline than the control groups （P<0.05）； the fatigue feel of observation group were obviously lower than the control group. All the study staff did not occur any adverse reactions， which showed that comfrey ex－ tract had obvious effect on alleviating sports fatigue， and had security.