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食品研究与开发:2017,38(4):158-161
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“藏灵菇”酸乳中优势乳酸菌的分离鉴定
柯晓静,于宏伟,郭润芳*
(河北农业大学 食品科技学院,河北 保定 071000)
Isolation and Identification of the Dominant Lactic Acid Bacteria in‘Tibetan Kefir’ Yogurt
KE Xiao-jing,YU Hong-wei,GUO Run-fang*
(College of Food Science and Technology, Agricultural University of Hebei, Baoding 071000, Hebei, China)
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投稿时间:2016-05-23    
中文摘要: 利用碳酸钙平板法从藏灵菇菌块为发酵剂制备的发酵酸乳中分离到 2 株产酸细菌, 对其进行形态、 生理生 化测定及 16S rRNA 序列分析。结果表明,发酵凝乳中的这两株优势乳酸菌是乳酸乳球菌乳酸亚种。本研究结果为开 发稳定的优良发酵剂提供菌种资源,并为进一步研究藏灵菇酸奶的保健功能奠定基础。
中文关键词: 藏灵菇  优势菌  乳酸菌  分离鉴定  16S rRNA
Abstract:Two acid-producing bacteria were isolated from the yogurt prepared by fermentation agents of Ti- betan kefir using calcium carbonate plate method. These two isolates were identified as Lactococcus lactis subsp. lactis based on the characterization of morphology, physiology and biochemistry, and 16S rRNA sequence analysis. The present research provide strain resources for fermentation agents, and also lay a foundation for fur- ther studying on health function of Tibetan kefir yogurt.
文章编号:201704035     中图分类号:    文献标志码:A
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