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投稿时间：2016-05-19
投稿时间：2016-05-19
中文摘要: 通过对检测条件的优化，制备高特异性、高稳定性、高敏感的 MIP-AuNPs-GCE 电化学传感器，建立新型的组 胺检测方法。通过对该传感器的电极电化学行为进行研究，分析出了电极电化学行为的变化规律，还对 AuNPs 的信 号放大作用进行了验证。 该方法的检测时间 < 30 min， 标准曲线为：Y = 2.953 lgX + 1.968，R2 = 0.998 8， 最低检测限 （LOD）：0.22 ng/mL， 检测范围：0.25 ng/mL~100 ng/mL。 以豆腐乳作为实际样品进行加标回收试验， 加标回收率位于
93.57 %~103.93 % 之间，相对标准偏差（RSD）位于 0.93 %~3.08 % 之间，各检测水平之间的差异具有统计学意义。
Abstract:By the optimization of the experimental conditions， a MIP-AuNPs-GCE electrochemical sensor was established which had a very good specificity， stability， and selectivity for histamine detection. The electrochem－ ical behaviors of the electrode were studied and its change regulation was obtained. The signal amplification effect of AuNPs was also verified. Electrochemical measurements were performed via electrochemical impedance spec－ troscopy（EIS）and the calibration curve obtained. The calibration curve formula was Y = 2.953 lg X + 1.968（R2 =
0.998 8）. The minimum detection limit of the established method was 0.22 ng/mL. The sensing technique allows for the detection of histamine in the wide range from 0.25 ng/mL to 100 ng/mL and detection time less than 30 min. The content of histamine in fermented bean curd samples were also detected using the spiked recoveries method. The determination results indicated good recoveries ， ranging from 93.57 % to 103.93 %， with relative standard deviation（RSD）of 0.93 % to 3.08 %. The detection results had a good statistical significance.
keywords: histamine electrochemical sensing technology molecularly imprinted polymer （MIP） films AuNPs signal amplification
文章编号：201704024 中图分类号： 文献标志码：A
基金项目:国家重大科学仪器设备开发专项（2013YQ140371）；国家自然 科学基金青年项目（21207161）；天津市科技支撑计划（14ZCZDSF00021）
| 作者 | 单位 |
| 姜随意1，吴业宾1，王浩1，宁保安2，白家磊2，彭媛2，高志贤2，* | 1. 解放军 61251 部队卫生队，河北 秦皇岛 066102；2. 军事医学科学院 卫生学环境医学研究所 天津市环境与食品安全风险监控技术重点实验室，天津 300050 |
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