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投稿时间：2016-03-09
投稿时间：2016-03-09
中文摘要: 利用酸性热水浸提法提取杨桃多糖，通过研究影响杨桃多糖提取率的 4 个因素：料液比、温度、提取时间、提 取次数进行单因素的研究。采用正交试验定了杨桃多糖最佳的提取工艺是：料液比为 1 ∶ 4（体积比），浸提时间是 3 h， 提取温度是 80 ℃，提取次数 3 次，各因素影响杨桃多糖提取率的次序是：温度>料液比>浸提时间>提取次数。杨桃多 糖的最佳的纯化工艺是：用无水乙醇沉淀多糖（多糖液与乙醇的体积比是 1 ∶ 5），活性炭脱色，Savage 法脱蛋白。最后 用透析袋进行透析得到灰白色粉末状的杨桃多糖。 杨桃多糖对羟基自由基的清除率随着杨桃多糖的浓度增大而增 大。清除羟基自由基的能力最高可达 51.49 %。当杨桃多糖的浓度达到一定时，多糖对羟基自由基的清除率受杨桃多 糖的影响不大。
Abstract:This study was the use of hot water extraction of acidic polysaccharides carambola， starfruit four fac－ tors polysaccharide extraction rate by studying effects： liquid ratio， temperature， extraction time， extraction times to study single factor. By orthogonal set of carambola best extraction of polysaccharides were： Solid-liquid ratio of 1 ∶ 4， extraction time was 3 hours， extraction temperature was 80 ℃， extracting 3 times， the factors af－ fecting the rate of the order of carambola polysaccharide are： temperature> liquid ratio> extraction time> extrac－ tion times. Best carambola polysaccharide purification process were： anhydrous ethanol precipitated polysac－ charide（multiple sugar and ethanol volume ratio was 1 ∶ 5）， active carbon， Savage Law deproteinized. Finally， dialysis bag and dialyzed to obtain a white powder carambola polysaccharide. Carambola polysaccharide hydrox－ yl radical scavenging rate increased as the concentration increases carambola polysaccharides. Ability to scav－ enge hydroxyl radicals was up to 51.49 %. When the concentration reached a certain carambola polysaccharide， the polysaccharide hydroxyl radical scavenging rate was not affected carambola polysaccharides.
文章编号：201704013 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| SONG Zhao-feng | Department of Chemistry and Chemical Engineering，Foshan University， Foshan 528000 Guangdong， China |
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