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食品研究与开发:2017,38(4):15-18
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木瓜蛋白酶水解大豆球蛋白的抗氧化性研究
邹险峰,陈丽娜,吴琼,怀影,张莉弘
(长春大学 食品科学与工程学院,吉林 长春 130022)
Study on Antioxidation of Papain Enzymolysis on Glycinin
ZOU Xian-feng, CHEN Li-na, WU Qiong, HUAI Ying, ZHANG Li-hong
(College of Food Science and Engineering , Changchun University, Changchun 130022, Jilin,China)
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投稿时间:2016-05-27    
中文摘要: 采用木瓜蛋白酶水解大豆球蛋白制备抗氧化肽。以自由基的清除率为评价指标,通过单因素试验和正交试 验确定酶解最佳条件。结果显示:最佳条件为 pH7.5,酶和底物比 8 000 U/g,水解时间 3 h,温度 60 ℃。优组合条件下的 羟自由基清除率为 77.6 %,超氧阴离子的清除率为 47.3 %。
Abstract:Glycinin was hydrolyzed by papain to produce antioxidant peptide. Base on the scavenging rate of radical, optimum enzymatic hydrolysis conditions were determined by single-factor and orthogonal test. Results showed that these optimum conditions were followed: pH 7.5, 8 000 U/g of enzyme concentration, 3 h of reaction time and 60 ℃. In these conditions, the scavenging rate of hydroxyl radical and superoxide anion were 77.6 % and 47.3 % respectively.
keywords: glycinin  antioxidation  papain
文章编号:201704004     中图分类号:    文献标志码:A
基金项目:吉林省教育厅“十三五”科学技术研究项目(2016296)
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