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食品研究与开发:2017,38(3):95-100
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红枣核总黄酮的提取工艺及抗氧化活性研究
范艳丽,张博,李梓溢,仝瑶,张航,翟刚
(宁夏大学,宁夏 银川 750021)
Study on Extraction Process and Anti-oxidative Activity of Total Flavonoids from Chinese Jujube Seeds
FAN Yan-li, ZHANG Bo, LI Zi-yi, TONG Yao, ZHANG Hang, ZHAI Gang
(Ningxia University, Yinchuan 750021, Ningxia, China)
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投稿时间:2016-05-14    
中文摘要: 采用 Box-Behnken Design(BBD)开展响应面试验,研究料液比、乙醇浓度、浸提温度、浸提时间和提取级数五因 素及其互作效应对红枣核总黄酮提取率的影响,确定红枣核总黄酮的最佳提取工艺条件为:料液比 1 ∶ 70(g/mL),乙醇 浓度 40 %,浸提温度 80 ℃,浸提时间 4 h,提取级数 3 次,在此条件下黄酮提取率为 16.64 mg/g。此外,以 BHT 和 VC 为阳性对照评价了提取物的抗氧化活性, 结果表明在一定浓度范围内红枣核总黄酮对超氧阴离子自由基、 羟基自由基、 DPPH 自由基、亚硝基离子自由基、ABTS 自由基的最高清除率分别达到 53 %、85 %、71 %、68 %、91 %,总还原能力分别 为 BHT 和 VC 的 35 %和 36 %,表明所提取的红枣核黄酮具有较强的体外抗氧化活性。
中文关键词: 红枣核  黄酮  提取  抗氧化
Abstract:The extraction effects of solid to liquid ratio, ethanol concentration, extraction temperature, extrac- tion time, extraction times and their interaction on total flavonoids from Chinese jujube seeds were studied using response surface methodology by Box-Behnken Design (BBD). The optimum extracting condition of total flavonoids was ascertained as solid to liquid ratio 1 ∶ 70(g/mL), ethanol concentration 40 %, extraction temper- ature 80 ℃, extraction time 4 h and 3 times, and the extraction rate of total flavonoids on this condition was 16.64 mg/g. Moreover, the antioxidant activity of the product was evaluated taking VC and BHT as positive con- trols. The results showed that the optimum scavenging rate of the product on oxygen free radical, hydroxyl radi- cals, DPPH free radicals, nitroso ion and ABTS radicals was 53 %, 85 %, 71 %, 68 % and 91 % respectively, and the total reductive ability was 35 % of BHT and 36 % of VC, revealing the significant antioxidant activity of the extracted flavonoids from Chinese jujube seeds in vitro.
文章编号:201703021     中图分类号:    文献标志码:A
基金项目:宁夏大学科学研究基金项目(ZR1437)
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