Abstract:By hot-melt method preparing chitosan-sodium caseinate composite film，adding natural antibacterial agent Natamycin and Lysozyme，made of edible antimicrobial film.by the infrared spectroscopy （FT-IR） characterised different antibacterial membrane structure and determinated the light transmittance， properties，UV absorbent and antimicrobial activity. The results showed that the components of different antimicrobial films have good interaction，in which the compatibility of M2 film was best；the mechanical properties of M1 film are the best，the tensile strength of M2 film was enhanced，and the absorption of UV light of the M1 and M3 was increased. The antibacterial effect of M2 membrane to the different strains was superior，especially for the fungus and yeast had the best antibacterial，and the difference reached significant level（P<0.05），the inhibition of bacteria for Pseudomonas fluorescens >Staphylococcus aureus >E.coil. The M2 film will have broad application prospects in cheese packaging.