Abstract:The pomegranate husk was the research subject， and the ethanol was used as extraction solvent， which is less toxic. Punicalagin was extracted as object from the pomegranate husk， and was tested by HPLC. Response surface methodology was used to optimize the extraction condition， and Box-behnken principle was used to design the test. By studying the scavenging rate of·OH，O2-·，DPPH·， the antioxidation in vitro of the punicalagin extract was assayed. The result of the optimization test of response surface methodology showed that the optimized condition for punicalagin extraction was： the content of ethanol solution was 60 %， the ratio of po－ mace to ethanol solution was 1 ∶ 25（g/mL）， and temperature was 60 ℃and time were 6 h， and in this condition the extracted punicalagin quantity could reach to 70.89 g/kg， and the sequence of each factor effecting on ex－ tracted punicalagin quantity was： the content of ethanol solution> temperature> time> the ratio of material to so－ lution； the free radical scavenging test showed that the punicalagin extract could scavenge·OH，O2-·，DPPH·very well， and exhibited better capacity of free radicals scavenging than the pomegranate husk.