Abstract:The head，bones，other raw waste of Cirrhinus molitorella were hydrolyzed by neutral protease，and response surface analysis method were used to optimize the enzymatic processes.The enzyme concentration，temperature，pH，solid to liquid ratio，time and other factors are discussed to analyze the effect of hydrolysis based on the degree of hydrolysis and peptide content.Three main factors and its three levels were chose recording to single factor test，then the experiment was based on the response surface design principles and Design-Expert software was used to analysis and validation.The results showed that：the optimum conditions of neutral hydrolysis：enzyme concentration5.1 mg/g，temperature55.16℃，time5.22 h，pH natrural，solid-liquid ratio 1∶5 （g/mL），the degree of hydrolysis and the peptide content is 13.01%and 1.07 mg/mL seperately in this condition.