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投稿时间：2015-09-25
投稿时间：2015-09-25
中文摘要: 为优化黑木耳蛋白质的碱溶酸沉法提取工艺，考察料液比、提取时间、提取温度、碱液浓度对黑木耳蛋白质得率的影响。采用响应面法优化黑木耳蛋白质提取的工艺条件。结果表明：黑木耳蛋白质的最佳提取工艺参数为：提取温度50℃、料液比1∶90（g/mL）、提取时间2.5 h、碱液浓度0.07 mol/L。在此最佳条件下，黑木耳蛋白质的得率为4.52%。由此可知，碱溶酸沉法可以较好地提取黑木耳蛋白质。
Abstract:In order to optimize the extraction process of protein from Auricularia auricular，solid-liquid ratio，extraction time，temperature and alkali concentration were investigated for their effects.The optimal extraction conditions were explored by response surface methodology.The results showed that optimal protein extraction parameters were temperature 50℃，solid-liquid ratio 1∶90（g/mL），extraction time 2.5 h，alkali concentration 0.07mol/L.Under these conditions，the yield of protein from Auricularia auricular was 4.52%.The results indicated that this protein extraction method was distinctly effective when applied on Auricularia auricular.
文章编号：201616009 中图分类号： 文献标志码：
基金项目:黑龙江省应用技术研究与开发计划项目（GA13B202）
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