Abstract:The liquid fermentation medium of Lepista sordida was optimized.We optimized carbon sources，nitrogen sources，pH，inorganic salt，fungus ages in medium with the biomass of mycelia as determining parameter by single factor analysis.The results showed that the best carbon source was to be the potato flour，the best nitrogen source was to be NH4Cl，the best pH was to be 7，the best inorganic salt was to be CuSO4and FeSO4in liquid fermentation medium.Meanwhile，the optimized of medium for liquid fermentation of Lepista sordida was screened through orthogonal experiment methods.The optimized formula of liquid fermentation medium were contained that carbon source was potato flour，nitrogen source was NH4Cl，pH=7，and inorganic salt was FeSO4，fungus ages was at 12 d.