Abstract:The method to simultaneously determinate six kinds of common mycotoxins，included nivalenol （NIV），deoxynivalenol concealed（Deepoxy-DON），deoxynivalenol（DON），deoxynivalenol derivatives（3ADON，15A-DON）and zearalenone（ZEN）in wheat flour was established.The samples were purified with multifunctional purifying column，then determinated by liquid chromatography tandem mass spectrometry with internal standard method.The results showed detection limits of six kinds mycotoxin were between 0.50 μg/kg and 1.00 μg/kg，the linear correlation coefficient R2＞0.994，three levels recovery were 82.1%-108.9%，RSD were 4.6%-14.5%.The method used to ability verification and quality control assessment of deoxynivalenol in wheat flour，statistical results Z score was-1.93，the result was evaluated as satisfactory.The method is accurate，sensitive and efficient and applicable to quantitative analysis of six kinds of common mycotoxin in wheat flour.