Abstract:Pleurotus ferulae was used as raw material.Water was used as extraction solvent.Ultrasonic microwave assisted extraction（UMAE）technology of polysaccharides from Pleurotus ferulae（PFP）was optimized by response surface methodology.The traditional water bath extraction（TWBE）was compared with UMAE.Antioxidant activity of PFP in vitro was evaluated by hydroxyl，superoxide anion and DPPH free radical scavenging method The optimum technology condition for ultrasonic microwave assisted extraction of PFP was determined as follows：the material-liquid ratio was 1∶50（g/mL），the extraction time was 10 min and microwave power was 60 W.Compared with the traditional water bath extraction，the ultrasonic microwave assisted extraction was time saving and increased the yield from 2.23%to 5.6%.UMAE had no effect basically on structure of PFP.PFP had strong scavenging effects of DPPH radical，hydroxyl radical and superoxide anion radical.Scavenging effects had the positive correlation with the concentration.when concentration of PFP reached 5 mg/mL，scavenging rate of DPPH radical，hydroxyl radical and superoxide anion radical was 67%，59%and 63%，respectively.However，Antioxidant activity of PFP was week compared with VC.