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食品研究与开发:2016,37(14):-
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米糠多糖铁的制备及其与ctDNA的相互作用
(1.武汉轻工大学生物与制药工程学院,湖北武汉430023;2.农产品加工湖北省协同创新中心,湖北武汉430023)
The Interaction of Rice Bran Polysaccharide Iron Complex and ctDNA
(1.School of Biological and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,Hubei,China;2.Hubei Collaborative Innovation Center for Processing of Agricultural Products,Wuhan 430023,Hubei,China)
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投稿时间:2015-07-01    
中文摘要: 在碱性条件下用米糠多糖与三氯化铁制备米糠多糖铁(RBPIC)。理化试验表明米糠多糖与三氯化铁生成了稳定的RBPIC,RBPIC在pH4.1~12.6的条件下可以稳定存在,RBPIC中铁含量为18.41%;通过紫外-可见吸收光谱研究了RBPIC与ctDNA的相互作用,结果表明RBPIC与ctDNA存在明显的相互作用,结合常数为7.27×106L/mol;琼脂糖凝胶电泳实验表明BRPIC/DNA可以有效促进DNA凝聚。
中文关键词: 米糠  多糖铁  ctDNA  紫外-可见吸收光谱
Abstract:Rice bran polysaccharide iron complex (RBPIC)was prepared with rice bran polysaccharides and ferric iron at alkaline conditions.RBPIC was stable at pH 4.1-12.6 and the iron content in RBPIC was 18.41%.The interaction between ctDNA and RBPIC was studied by UV-vis absorption spectra.The results showed that the interaction between ctDNA and RBPIC was obvious,and the binding constant was 7.27×106L/mol.
文章编号:201614037     中图分类号:    文献标志码:
基金项目:湖北省教育厅科学研究计划项目(B2016077);武汉轻工大学大学生科研项目(xsky2015008);武汉轻工大学2015校立科研项目(2015d5)
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