Abstract:Cronobacter sakazakii is an emerging pathogen which causes a life-threatening syndrome of meningitis，necrotizing colitis and meningoencephalitis in neonates and infants.Biofilm formed on the surface of silicone is an important possible source of clinical infections caused by this pathogen.Crystal violet staining method was employed to determine biofilm maturation curves of C.sakazakii ATCC BAA-894 on silicone.The processes of static biofilm formation on the silicone were investigated using the scanning electron microscope（SEM）.The bacteria on the surface had been observed to aggregate and grow into microcolonies，form 3-dimensional structures and communal relationships，resulting in a complex biofilm.In addition，the SEM revealed biofilm morphologies with variable amounts of extracellular polymeric substances，the presence of microcolonies and the mature biofilm formed between 12 h and 24 h.