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投稿时间：2015-07-19
投稿时间：2015-07-19
中文摘要: 以乙醇为提取溶剂，采用单因素和响应面分析法相结合的手段优选芹菜中黄酮提取工艺。首先通过单因素试验初步探讨了液料比、乙醇浓度、提取温度和提取时间4个主要因素对黄酮得率的影响规律。然后，采用Box-Behnken中心组合设计试验，建立了回归方程的预测模型，方差和响应面分析结果表明：4个因素及其二次项对黄酮提取率影响显著，料液比和提取温度、料液比和提取时间、乙醇体积分数和提取温度之间的交互作用显著。最终，确定了芹菜黄酮提取的最佳工艺条件为液料比41∶1（mL/g），乙醇体积分数84%，提取温度83℃，提取时间134 min，在此条件下黄酮提取率为3.276%。
Abstract:The optimal extraction process of flavonoids from celery were investigated using ethanol as solvent by single factor experiment and response surface methodology.The effects of liquid-solid ratio，ethanol concentration，extraction temperature and extraction time on the flavonoids yield were discussed.Further，the predictive model of regression equation was established by Box-Behnken design.The results of Anova and response surface analysis indicate that，the flavonoids yield was significantly influenced by the 4 factors and their quadratic term.And the interactions were prominent among liquid-solid ratio，extraction temperature and extraction time，ethanol concentration and extraction temperature.Ultimately，the optimum extraction conditions were obtained as follows：liquid-solid ratio 41 ∶1 （mL/g），ethanol concentration 84%，extraction temperature 83 ℃ and extraction time 134 min.Under these conditions，the extraction rate is 3.276%.
文章编号：201614013 中图分类号： 文献标志码：
基金项目:徐州工程学院校级科研课题（XKY2013316）
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