女贞花总黄酮提取及清除DPPH自由基作用的研究

姚文红1; 李飞阳2; 王娟1; 骆珍1; 侯婷1; *

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DOI:10.3969/j.issn.1005-6521.2016.14.012

食品研究与开发:2016,37(14):-

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女贞花总黄酮提取及清除DPPH自由基作用的研究

姚文红1，李飞阳2，王娟1，骆珍1，侯婷1，*^1,2

(1.青岛农业大学，山东青岛266109;2.山东师范大学，山东济南250014)

Study on Extraction of Total Flavonoids in Ligustrum Flowers and Its Scavenging Activity on DPPH Free Radicals

YAO Wen-hong1，LI Fei-yang2，WANG Juan1，LUO Zhen1，HOU Ting1，*^1,2

(1.Qingdao Agricultural University，Qingdao 266109，Shandong，China;2.Shandong Normal University，Jinan 250014，Shandong，China)

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投稿时间：2015-07-23

中文摘要: 在单因素试验的基础上，利用正交试验优化提取女贞花中总黄酮的工艺条件，并研究对DPPH自由基清除作用。结果表明：以0.5 g待测品为原料，按照1∶55（g/mL）料液比加入50%乙醇为提取剂，85℃恒温回流提取60 min为最佳提取工艺，女贞总黄酮提取率为12.57%（RSD=0.74）；女贞黄酮对DPPH自由基清除率IC50值为11.97 μg/mL，最大清除率82.06%。

中文关键词: 女贞花总黄酮 DPPH自由基

Abstract:To optimize the extraction of total flavonoids in Ligustrum flowers and its scavenging activity on DPPH free radicals，the factors influencing the process parameters were determined by specotrophotometric method.The results showed the optimum conditions of the extraction were as followes：0.5 g of samples were used as the raw material，50%ethanol solution was used as the extraction solvent with a raw material to extraction solvent ratio of 1∶55（g/mL），and the reflux extraction was carried out in 85℃water bath for 60 min.The extraction rate was 12.57% （RSD=0.74）.The IC50value of DPPH free radical scavenging rate by flavonoids in Ligustrum flowers was 11.97 μg/mL，and the maximum the clearance rate of DPPH free radical was 82.06%.

keywords: Ligustrum flowers total flavonoids DPPH free radical

文章编号：201614012 中图分类号： 文献标志码：

基金项目:青岛农业大学高层次人才科研基金（663-1113320）；青岛农业大学大学生科技创新基金（2014305）

作者	单位
姚文红1，李飞阳2，王娟1，骆珍1，侯婷1，*	青岛农业大学，山东青岛266109；2.山东师范大学，山东济南250014

Author Name	Affiliation
YAO Wen-hong1，LI Fei-yang2，WANG Juan1，LUO Zhen1，HOU Ting1，*	Qingdao Agricultural University，Qingdao 266109，Shandong，China；2.Shandong Normal University，Jinan 250014，Shandong，China

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