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投稿时间：2015-11-27
投稿时间：2015-11-27
中文摘要: 以多酚、黄酮、三萜和维生素C 4种活性物质为指标，研究超声时间、超声温度、料液比和超声功率对忧遁草中活性物质提取效果的影响，利用正交试验优化超声辅助提取忧遁草活性物质的最佳工艺条件。试验结果表明料液比和超声时间对多酚、黄酮和维生素C得率的影响较大；超声功率和超声时间对三萜得率影响较大。通过四因素三水平的正交试验，确定了忧遁草中4种活性物质提取的最佳条件，得出超声辅助提取的最优提取工艺是：超声时间40 min，超声温度45℃，料液比1∶55（mg/mL），超声功率156 W。在此条件下4种活性成分的得率分别为：多酚9.387 mg/g，黄酮28.785 mg/g，三萜23.665mg/g，维生素C0.216mg/g。
Abstract:With polyphenols，flavonoids，triterpenoid and vitaminC as detection index，the effect of ultrasonic temperature，time，power and solid-liquid ratio on extraction efficiency was studied.Optimum extraction process was determined by orthogonal test.The solid-liquid ratio and ultrasonic time had great effect on extraction efficiency of polyphenols，flavonoids and vitaminC.The ultrasonic power and ultrasonic time had a greater effect on triterpenoid than the temperature and solid-liquid ratio.The best condition of active substance of Clinacanthus nutans was determined by four-factor and three-level orthogonal test.The optimal extraction process conditions were determined as follows：ultrasonic temperature，45℃；ultrasonic power，156W；and solid-liquid ratio，1∶55（mg/mL）for an extraction duration of 40 minutes.Under these conditions the extraction yield of each component were：polyphenols 9.387 mg/g，flavonoids 28.785 mg/g，triterpenoid 23.665 mg/g，vitaminC 0.216 mg/g.
文章编号：201613040 中图分类号： 文献标志码：
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