本文已被：浏览 302次 下载 0次
投稿时间：2016-03-27
投稿时间：2016-03-27
中文摘要: 采用高效液相色谱串联质谱联用技术（HPLC-MS/MS）测定了几种高蛋白食品中羧甲基赖氨酸（CML）的含量，包括：液态牛奶，奶粉，饼干，面包和蛋白类发酵食品——酱油。对样品进行还原、沉淀、水解以及除杂后上样检测，对固相萃取柱及洗脱液进行了优化，采用外标法定量，HPLC-MS/MS的分析时间为25 min。其中酱油和饼干中CML的含量最高达到（385.59±7.73）mg/kg蛋白和（491.75±3.13）mg/kg蛋白，奶粉中CML的含量是最低的（45.66±2.89）mg/kg蛋白。
中文关键词: 羧甲基赖氨酸 高效液相色谱串联质谱联用技术 食品 含量测定
Abstract:High performance liquid chromatography-mass spectrometry（HPLC-MS/MS）was used to detect Nε-（carboxymethyl）lysine（CML）in several foods which contain high protein content，including liquid milk，powdered milk，biscuit，bread and soybean sauce.Samples were analyzed before pre-processing with sodium borohydride reduction，protein isolation，hydrolysis，and removing impurity.The optimization of different solid phase extraction cartridges and eluents in this study，and the analysis time of HPLC-MS/MS was 25 min.The CMLlevel in foods were as follows，soybean sauce and biscuit had the highest content，up to（385.59±7.73）mg/kg pro and（491.75±3.13）mg/kg pro，powdered milk had the least content，up to（45.66±2.89）mg/kg pro.
keywords: Nε-（carboxymethyl）lysine high performance liquid chromatography-mass spectrometry food determination of content
文章编号：201613019 中图分类号： 文献标志码：
基金项目:国家自然科学基金（31501559；31571940）
引用文本：
津ICP备20000603号-1