Abstract:Researching the anti-fatigue effect of Oviductus Ranae protein hydrolyzate.Using double enzyme to prepare Oviductus Ranae protein hydrolyzate.Four doses of Oviductus Ranae protein hydrolyzate with low，medium and high dose group and a blank control group were applied to mice continuously for 30 d.Then measured loaded swimming time，serum urea content，blood lactic acid content and hepatic glycogen of mice.Compared with blank control group，the loaded swimming time of mice of each dose group was significantly prolonged（P＜0.05），the serum urea content after swimming of mice of high and middle dose groups were both significantly lower（P＜0.05），the areas under the curve of the three time points of blood lactic acid after swimming of mice of high dose group were lower than the blank control group（P＜0.05），the hepatic glycogen content of each dose group was no significant differences（P＞0.05）.Oviductus Ranae protein hydrolyzate had the effect of relieving physical fatigue.