Abstract:Flammulina velutipes scraps was used as raw materials.Polysaccharides from Flammulina velutipes scraps（FVSP）were extracted with water.Deprotein rate，polysaccharide retention rate and decoloration rate were used as the index.Decolorization technology of FVSP by polyamide chromatography was investigated.Antibacterial activity of FVSP was also explored.The results showed that polyamide chromatography was the best deproteinization method on FVSP.The optimum decolorization technology was as follows：the content of polyamide was 50 g，and the polyamide column was eluted with 1.5 column volumes of deionized water at flow rate of1.5 mL/min.Under this technology，the deprotein rate was 94.2%.FVSP had no antimicrobialactivity of Aspergillus niger and Saccharomyces cerevisiae and had antibacterialactivities against Staphylococcus aureus，Escherichia coli and Bacillus subtilis with minimum inhibitory concentration of2.25，5，2.25 mg/mL，respectively．The antibacterialactivity was positively correlated with polysaccharides concentration.