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投稿时间：2013-09-28
投稿时间：2013-09-28
中文摘要: 采用水提醇沉法提取新疆沙枣多糖，并采用DM-18型大孔树脂对粗沙枣多糖进行分离纯化，确定了最佳的分离条件。通过粗沙枣多糖和纯化后的沙枣多糖对DPPH自由基、羟基自由基（·OH）、超氧阴离子自由基（O2－·）的清除能力进行比较。结果表明：在沙枣多糖样品溶液2.0 mg/mL，上样速率为1.5 BV/h，上样液pH值为7.0，上样量为3.0 BV、洗脱剂乙醇浓度为35%、洗脱剂用量为3.0 BV、洗脱速率为1.0 BV/h时，洗脱剂pH值为8时，DM-18型大孔树脂对沙枣多糖的动态吸附率和解吸率分别达到90.23%和92.37%，粗沙枣多糖的纯度为42.33%，纯化后的多糖纯度为70.56%。粗多糖和纯化后多糖对DPPH·、·OH、O2－·均有清除作用，纯化后多糖的抗氧化活性大于粗多糖。
Abstract:Polysaccharide from Elaeagnus angustifolia in Xinjiang was extracted by water-alcohol precipitation. DM-18 macroporous resins were used to isolate the crude polysaccharide，the best condition was confirmed. Antioxidant activities of the crude and purified polysaccha ride were evaluated through their capability of scavenging DPPH·，·OH，O2－·.The result showed that best conditions as follows：2.0 mg/mL of concentration and 7.0 of pH of the solution of Elaeagnus angustifolia polysaccharide，the sample loading amount of 3.0 BV，1.5 BV/h ofthe rate，the eluent concentration of35%，the total quantity of eluentof 3.0 BV，8 ofpH ofthe solution and eluent rate of 1.0 BV/h.The rates of absorption and desorption could achieve 90.23% and 92.37% under the conditions above.The purity of the crude polys accharide and the purified polysac charide were 42.33%and 70.56%.Both ofthe polysaccharide crude and purified had significanteffecton scavenging DPPH·，·OH，O2－·. Further more purified polysac charide had more activity antioxidant than crude polysac charide.
keywords: Elaeagnus angustifolia polysac charide macroporous resins separate purificate antioxidation
文章编号：201612009 中图分类号： 文献标志码：
基金项目:教育部国际合作项目“春晖计划”（Z2006－1－83003）
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