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投稿时间:2015-06-22
投稿时间:2015-06-22
中文摘要: 研究麻疯树果壳多糖的提取工艺条件及抗氧化活性。采用麻疯树果壳为原料,以多糖得率为指标,在单因素的基础上,通过正交试验,研究了提取温度、提取时间、料水比和乙醇浓度4个因素对麻疯树果壳多糖得率的影响,并对其抗氧化活性进行研究。结果表明,麻疯树果壳多糖的最佳提取工艺条件为:温度90℃,时间2.5 h,料水比1∶20(g/mL),乙醇体积分数为90%,在此条件下麻疯树果壳多糖得率为5.75%。乙醇体积分数对麻疯树果壳多糖得率的影响程度达显著水平,而提取温度、料水比和提取时间对麻疯树果壳多糖得率没有显著的影响。麻疯树果壳多糖具有较好的清除·OH和DPPH·的能力,且其具有一定的还原能力。
Abstract:The extraction technology and antioxidation activity of polysaccharides from Jatropha curcas Fruit Shell were studied.The content of polysaccharides was determined using fruit shell of jatropha as raw material. On the basis ofsingle factor test,the effects of four factors including temperature,time,solid-liquid ratio and ethanolconcentration on polysaccharide yield were studied through or thogonal experiment design.Besides,antioxidant activity of the polysac charides was studied in this paper.The established optimum technological parameters were temperature 90℃,extraction time 2.5 h,solid-water ratio1∶20(g/mL),and ethanolcon centration 90%.The yield of polysac charides was 5.75%under the opticalconditions.The ethanol concentration had significant effecton polysac charide yield,and extraction temperature,solid-water ratio and extraction time had no signifi cant effect.The polysac charides of fruitshell of jatropha showed good capabilities to scavenge·OH and DPPH·with certain reducing power activity.
文章编号:201612010 中图分类号: 文献标志码:
基金项目:广西高等学校优势特色专业建设项目(桂教高教[2014]52号);广西教育厅科研项目(201203YB171;KY015YB278);广西高校桂西生态环境分析和污染控制重点实验室开放基金(1215KF09);广西林产化学与工程重点实验室开放基金(GXFC13-07)
作者 | 单位 |
张金磊1,李鹏飞2,马博1,赵丹丹1,全青敏1,雷福厚2 | 广西高校桂西生态环境分析和污染控制重点实验室百色学院,广西百色533000;2.广西民族大学广西林产化学与工程重点实验室,广西南宁530006 |
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