Abstract:We established a rapid detection method for proteus by using real-time fluorescence loop-mediated isothermal amplification（RealAmp）technology.Four loop-mediated isothermal amplification primers were constructed based on the atpD gene of proteus published in GenBank.The portable fluorescence reader（ESEQuant Tube Scanner）was used for isothermal（62°C）amplification of DNA template.Real-time monitoring was achieved by connecting the reader to a computer.Sensitivity，specificity and the detection limit of artificially contaminated pork of proteus were determined using RealAmp and compared with the sensitivity of the conventional LAMP.The results showed that RealAmp method was more simple and rapid than the conventional LAMP.The total time of judgment result of the former was generally less than 20 min.A total of 19 strains were subjected to specificity tests，6 proteus strains indicated positive results，whereas the other 13 non-proteus strainsindicatednegativeresults.ThesensitivityofRealAmpindetectingpureculturesof proteuswas8.1CFU/mL，which was10 times compared with that of the conventional LAMP.Moreover，the detection limit of artificially contaminated pork was 81 CFU/mL.The RealAmp method was superior in terms of testing time，convenience，specificity，and sensitivity，and had significant potential for rapid detection of proteus.