Abstract:Reversed-phase high performance liquid chromatography was used to analyze sugars in apples，in light of the characteristics of sugars，the adjusted high performance liquid chromatography technique was employed to analyze sugars.The chromatographic conditions：octadecylsilyl column，refractive index detector，column temperature at 25℃，injection volume was 10 μL，a mobile phase was Vmethanol∶Vwater=3∶97，flowing rate was 1 mL/min，isocratic elution.Five main sugars in apples were detected under the selected conditions：fructose，glucose，sucrose，arabinose and D-xylose；sugar compositions were found little difference among the varieties of Guoguang，Fushi，Hongyu and Huangjin bought on the market.