Abstract:Explored the substance of blueberry wine on delaying lifespan of C.elegans.The lifespan assay was carried out on model organism C.elegans.Base on blueberry wine，compounded six feeding group（0，1，2，3，4，5 mg/mL）by add different quality of PC，and establish seven feeding group（0%，5%，10%，15%，20%，25%，30%）by add different volume alcohol.Tested the lifespan，reproductive capacity and movement capacity on C.elegans.Feeding the alcohol content of 14%，compared with control group，the lifespan of C.elegans prolonged with promotion of PC concentration of blueberry wine，lifespan of C.elegans longer after 3mg/mL（P＜0.05）；it had different degrees of enhancement effect to the reproductive ability and movement ability（P＜0.05），while the increasing trend in not obvious when PC≥5mg/mL.Shorten the lifespan of C.elegans，accelerate the C.elegans death after feeding the 3 mg/mL of PC，different alcohol content of blueberry wine，its effect was positively related with alcohol content.The reproductive ability and movement ability weakened when alcohol is the single factor test.PC of blueberry wine have the function of the delay lifespan of C.elegans，concentration in the 3 mg/mL to 5 mg/mL obviously；alcohol of blueberry wine increased accumulation will accelerate the death of C. elegans.