Abstract:Inhibitory effect of polysaccharides from Boschniakia rossica（BRPS）on cellular oxidative stress induced by hydrogen peroxide（H2O2）in HepG2 cell line was investigated.The cellular oxidative stress model was established in HepG2 cells with H2O2，and then the cell viabilities were detected with MTT assay.Lactate dehydrogenase（LDH），alanine aminotransferase（ALT），aspartate aminotransferase（AST），malondialdelyde （MDA），superoxide dismutase（SOD）and reduced glutathione（GSH）were measured by the spectrophotometric method.The activation of extracellular signal-regulated kinase（ERK），c-Jun N-terminal kinase （JNK），p38 mitogen-activated protein kinase（p38）were determined with the western blotting method.Results showed that BRPS had no toxic effect on cultured HepG2 cells at the concentrations of 12.5 mg/L-50 mg/L. However，H2O2decreased the cell viability and antioxidant activities，and increased activation of ERK and JNK.In HepG2 cells with H2O2-induced oxidative stress，the administration of BRPS increased the cell viability，reduced LDH，ALT and AST leakage，reduced the MDA formation，increased the SOD and GSH levels，and suppressed the activation of ERK and JNK.Taken together，BRPS had the inhibitory effect on oxidative stress induced by H2O2in HepG2 cells，and could relieve the oxidative damage of HepG2 cells.