Abstract:For fast，effective detection of Shigella in milk，a strong specificity，high sensitivity real-time PCR method was established.The specific primers were designed according to the conserved sequence of Shigella ipaH gene published in GenBank，the extracted Shigella DNA template was amplified by PCR，the target gene was cloned and sequenced，then using the fluorescent quantitative PCR method，to detect Shigella，determine the amplification conditions.The established method specificity is high，the detected sensitivity reach 10-6ng/μL. Using the method of Shigella in milk can be detected at 28.4 cfu/mL.The method provides a reference for fast，accurate detection Shigella in milk.