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投稿时间：2015-03-16
投稿时间：2015-03-16
中文摘要: 建立樟芝粗多糖的含量测定方法。以葡萄糖为对照品，采用 3，5-二硝基水杨酸比色法，测定樟芝中还原糖和总 糖的含量，并计算出总多糖的含量。研究结果表明多糖含量在 0.25 mg/mL～1.00 mg/mL 范围内标准曲线具有良好的线性 关系，测得回归方程 y=0.597 9x-0.037 8（R2=0.999 6）。还原糖和总糖的平均加样回收率分别为 101.65 %（RSD=1.89 %）和 100.92 %（RSD=2.45 %）。樟芝中总多糖含量为 129.5 mg/g～148.1 mg/g。本方法灵敏、稳定、重复性好，有助于樟芝质量控制 方法的研究。
中文关键词: 樟芝 多糖 3，5-二硝基水杨酸比色法
Abstract:A method for the determination of polysaccharide in Antrodia cinnamonea （AC） was developed.To calculate the total polysaccharide content with the reducing sugar and total sugar content that was detemined by 3，5- dinitrosalicylic acid colorimeitry with glucose as the standard substance. The results showed that：The cali－ bration curve of polysaccharide was in good linearity over the range of 0.25 mg/mL-1.00 mg/mL，the regression equation was y=0.597 9x-0.037 8（R2＝0.999 6）.The average recovery of reducing sugar solution and total sugar solution in the test were 101.65 % with RSD，1.89 % and 100.92 % with RSD，2.45 % respectively.The polysac－ charide content of AC was 129.5 mg/g-148.1 mg/g.The method which can be used for the quality control of AC is convenient，accurate and sensitive with good reproducibility．
文章编号：201609038 中图分类号： 文献标志码：A
基金项目:深圳市战略新兴产业发展专项资金项目（CYZZ20130416170923767）
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