Abstract:The extraction of selenium and combined of selenium in selenium-riched tea were studied. Protein combined of selenium was extracted by hydrochloric acid， polysaccharide combined of selenium and nucleic combined of selenium were extracted by removing protein combined selenium with [（1 ∶ 1 phenol-methylene chloride）-isoamyl alcohol]（24 ∶ 1，mL/mL）. The selenium content of tea and nucleic， polysaccharide and pro－ tein combined of selenium were determined by atomic fluorescence spectrometry. The results showed that： Seprotein and Se-polysaccharide accounted for about 51.65 % and 25.23 % of the total selenium， the most impor－ tant factors influencing the dissolution rate of selenium from tea was the soaking temperature， the dunking time and frequency. The dissolution rate was 46.35 % at 95 ℃ ， and soaking three minutes， the dissolution rate of selenium was 25.80 %. Content of selenium could not be detected when dunked three times. The selenium ex－ tracted from tea is almost organic selenium， selenium -protein >selenium -polysaccharide >selenium -nucleic. Socking tea should be three minutes and no more than two times at 95 ℃.