Abstract:Glucoamylase， is produced by a special strain of Aspergillus niger， has been widely used in fermen－ tation industry of glucose， amino acids， antibiotic， etc. This paper aims to evaluate the yield and activity of glucoamylase which produced by modified strain and starting strain respectively by means of molecular modifi－ cation technology， such as mutation breeding， gene knockout， RNA interference and gene recombinant expres－ sion， etc. With Aspergillus niger starting strain as the research material and gene modification as the research background， in order to explore the excellent means of molecular modification on Aspergillus niger for high-yield glucoamylase， this paper provides technical support for later research on the modification of Aspergillus niger with new efficiency and better yield.