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食品研究与开发:2016,37(8):99-103
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海洋低温α-淀粉酶酶解豌豆淀粉及其生物活性
吕明生1,2,王淑军1,2,焦豫良1,2,房耀维1,2,刘姝1,2,薛晔敏1
(1. 淮海工学院 海洋学院,江苏 连云港 222005;2. 淮海工学院 江苏省海洋生物产业技术协同创新中心, 江苏 连云港 222005)
Hydrolyzate of Pea Starch by Marine Cold-adapted α-Amylase and Its Biological Activity
Lü Ming-sheng1,2, WANG Shu-jun1,2, JIAO Yu-liang1,2, FANG Yao-wei1,2, LIU Shu1,2, XUE Ye-min1
(1. School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, Jiangsu, China; 2. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, Jiangsu, China)
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投稿时间:2015-02-06    
中文摘要: 豌豆淀粉是一种广泛用于食品加工的重要的食品原料,采用研制的新型海洋低温 α-淀粉酶,结合单因素试验 和正交试验优化了 α-淀粉酶酶解豌豆淀粉的工艺参数,并对酶解产物的生物活性进行了研究。结果表明,低温 α-淀粉 酶酶解豌豆淀粉工艺条件为加酶量 35 U/g,时间 165 min,温度 35 ℃,豌豆淀粉浓度 4 %,pH6.5。经高效液相色谱(HPLC) 分析,酶解产物中麦芽三糖、麦芽四糖和麦芽五糖的总和达到 65.88 %。酶解产物对羟自由基和 DPPH 自由基均有清除 作用,对 DPPH 自由基的清除效果好于对羟自由基的清除效果。为豌豆淀粉的深入加工提供了参考。
Abstract:Pea starch has become an important food ingredient and has been widely used in food processing. Some factors which could influence enzymatic hydrolysis were studied. The optimal conditions of pea starch hy- drolysis with the α-amylase: enzyme amount 35 U/g, hydrolysis time 165 min, temperature 35 ℃, pea starch concentration 4 %, and pH 6.5. The amylase could hydrolyze pea starch into maltose, maltotriose, maltote- traose and maltopentaose by HPLC of analysis. The quantity of maltotriose, maltotetraose and maltopentaose was 65.88 %. The hydrolyzate had the scavenging effect on the hydroxyl radical and DPPH radical. The scav- enging effect of DPPH radical was higher than one of hydroxyl radical.
文章编号:201608024     中图分类号:    文献标志码:A
基金项目:国家海洋公益性行业科研专项(201205020-8);国家自然科学基金(31271929)
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