本文已被：浏览 2074次 下载 682次
投稿时间：2015-01-09
投稿时间：2015-01-09
中文摘要: 探究石仙桃的总黄酮的提取方法的比较。以乙醇-水为提取体系，分别采用回流法、超声法，探究乙醇浓度、料 液比、提取时间等对石仙桃中总黄酮提取的影响，确立最佳条件。通过正交试验证明，超声波辅助提取的最佳工艺为：温 度 40 ℃，料液比 1 ∶ 40（g/mL），提取时间 50 min，在此条件下提取的黄酮含量为 26.12 mg/g；乙醇回流法提取最佳工艺为： 温度 60 ℃，料液比 1 ∶ 50（g/mL），提取时间 50 min，在此条件下提取的黄酮含量为 22.03 mg/g。超声波辅助提取法提取石 仙桃中总黄酮的提取率高于乙醇回流提取法提取。
Abstract:A comparative study of the extraction methods of the total flavonoids of Pholidota chinensis Lindl. was researched.With ethanol-water as the extraction medium，the reflow，ultrasonic methods were used in the extraction of the total flavonoids of Pholidota chinensis Lindl.. By exploring the ethanol concentration，solid-liq－ uid ratio，extraction time，etc，the optimum extraction conditions were obtained. The optimal extraction condi－ tions of flavonoids by ultrasonic assisted extraction method were extraction time for 50 min，solid to liquid ratio at 1 ∶ 40（g/mL），and extraction temperature at 40 ℃．Under the optimum conditions，the content of extracted to－ tal flavonoids from Pholidota chinensis Lindl. was 26.12 mg/g.The optimal extraction conditions of flavonoids by ethanol reflux extraction method were extraction time for 50 min，solid to liquid ratio at 1 ∶ 50 （g/mL），and ex－ traction temperature at 60 ℃．Under the optimum conditions，the content of extracted total flavonoids from Pholi－ dota chinensis Lindl. was 22.03 mg/g. The extraction rate of the total flavonoids of Pholidota chinensis Lindl. by ultrasonic assisted extraction method was higher than by reflux extraction method．
keywords: Pholidota chinensis Lindl. total flavonoids reflux extraction ultrasonic assisted extraction
文章编号：201607015 中图分类号： 文献标志码：A
基金项目:江西省自然科学基金（2012ZBA20513）；赣南医学院产学研项目（201205）
引用文本：
津ICP备20000603号-1