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食品研究与开发:2016,37(6):55-58
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牛奶中超氧化物歧化酶提取工艺研究
张立娟,张斌,任亚妮,王玥玮,温禄云,王麒琳
(天津市食品研究所有限公司,天津 301609)
Study on Extraction of Superoxide Dismutase in Milk
ZHANG Li-juan, ZHANG Bin, REN Ya-ni, WANG Yue-wei, WEN Lu-yun, WANG Qi-lin
(Tianjin Food Research Institute Co., Ltd., Tianjin 301609, China)
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投稿时间:2015-06-25    
中文摘要: 从牛奶中提取超氧化物歧化酶(SOD),通过单因素和正交试验,考察了加水倍数、葡萄糖酸铜添加量、葡萄糖酸 锌添加量、乙醇添加量、pH、第一次和第二次静置时间 7 个工艺参数对提取的 SOD 活性的影响。得出的最佳提取条件 为:蒸馏水与牛奶比例 2 ∶ 1(体积比),葡萄糖酸铜添加量为牛奶量的 4 %,葡萄糖酸锌的添加量为牛奶的量 3 %,加入牛 奶与蒸馏水总量 2 倍的冷乙醇, pH 调至 7.5,第一次静置 5 h,第二次静置 5 h。此条件下提取得到的 SOD 的活性为 127 623 U/g。
Abstract:The superoxide dismutase (SOD) extracting method from milk was investigated by single factor and orthogonal experiment. The effects of the ratio of milk to water, pH, the first standing time, the second standing time, the additive amount of alcohol, copper gluconate and the zinc gluconate on the SOD activity were studied. The optimal extracting condition was water and milk ratio 2 ∶ 1, copper gluconate 4 % of the amount of milk, zinc gluconate 3 % of the amount of milk, cold alcohol tow times of milk and water, pH 7.5, the first standing time 5 h, the second standing time 5 h. In such condition the SOD activity was 127 623 U/g.
文章编号:201606013     中图分类号:    文献标志码:A
基金项目:天津市科研院所技术开发工作扶持经费项目(YSK2012-23)
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