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投稿时间：2015-05-22
投稿时间：2015-05-22
中文摘要: 为了获得高效通用的水果果肉 DNA 提取方法，本研究以 11 种水果为试验材料，从前处理方法、裂解液成分、异 丙醇沉淀时间等方面对 CTAB 法进行了改进，并比较了行业标准法、试剂盒法、改良 CTAB 法 3 种方法的提取效果，发 现改良 CTAB 法提取的水果果肉 DNA 浓度都在 46.25 μg/mL 以上，纯度都在 1.8 左右，且能扩增出 137 bp 的 18SrDNA 条带，从而确定了高效通用的水果果肉 DNA 提取方法:改良 CTAB 法。
Abstract:In order to obtain an efficient and general DNA extraction method from fresh fruit，eleven kinds of fruit were taken as experimental material，the CTAB method was developed from several aspects，such as the pre -treatment methods，the composition of lysis buffer，the time of Isopropyl alcohol precipitation and so on. Furthermore，the three methods were compared on their extraction efficiency which included industry standard method，DNA extraction kit method and modified CTAB method. The results showed that modified CTAB method was the most suitable for the DNA extraction from fresh fruit，the concentration of isolated genomic DNAs was in more than 46.25 μg/mL，the value of OD260 / OD280 was close to 1.8，and the isolated genomic DNAs also amplified the 137 bp size banding of endogenous 18SrDNA .
keywords: fruit DNA extraction modified CTAB method
文章编号：201605014 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| QI Ling-qian，LIU Xiu*，DING Meng-xuan，LIU Yuan-yuan，KE Run-hui，YIN Jian-jun | China National Research Institute Food & Fermentation Industries，Beijing 100015， China |
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