Abstract:To optimize the extraction technology of polysaccharides from Herba Taraxaci，contrast experiment were carried out. The single factor experiment and Box-Behnken experiment were designed to study the effects of the pH， enzymolysis time，enzyme solution temperature， enzyme concentration on the extraction yield by enzymatic hydrolysis . On the basis of the type selection of enzyme， the optimum parameters of Herba Taraxaci polysaccharide extraction yield was determined. Results analysis of variance showed that enzyme concentration could have a greater impact on the extraction rate of polysaccharides， then was enzymolysis time and enzyme solution temperature，the last was the pH. The extraction yield by papaya protease was the highest（3.11 %）；The optimal conditions of extraction by enzymatic hydrolysis were:pH 9.20，enzyme solution temperature 46.18 ℃， enzymolysis time 123 min，enzyme content 3.38 % （enzyme quality/quality of dandelion）.