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食品研究与开发:2016,37(5):4-7
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鹰嘴豆水提物对α-葡萄糖苷酶的抑制作用研究
赵堂彦1,孟茜1,王琴2,张奇波2,陈霞2,黄玉军2,陈大卫2,顾瑞霞1,*
(1. 扬州大学 江苏省乳品生物技术与安全控制重点实验室,江苏 扬州 225127;2.新疆克拉玛依农牧业 科学技术研究所,新疆 克拉玛依 834000)
Study on the Inhibiting Effects of the Aqueous Extract from Chickpeas on the α-Glucosidase
ZHAO Tang-yan1,MENG Qian1,WANG Qin2,ZHANG Qi-bo2,CHEN Xia2,HUANG Yu-jun2,CHEN Da-wei2,GU Rui-xia1,*
(1. Jiangsu Province Key Laboratory of Dairy Biotechnology and Safety Control,Yangzhou University, Yangzhou 225127,Jiangsu,China;2. Xinjiang Karamay Agriculture Science and Technology Research Institute,Kelamayi 834000,Xinjiang,China)
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投稿时间:2015-10-30    
中文摘要: 研究鹰嘴豆水提物的不同组分对 a-糖苷酶的抑制作用,其对 a-糖苷酶的半抑制浓度为 0.20 mg/mL。将鹰嘴 豆水提物经过大孔树脂 D101 筛选,对其不同组分进行活性评价。结果发现鹰嘴豆水提物经过不同乙醇浓度洗脱,其 对 α-糖苷酶的抑制效果逐渐增强,其中 40 %乙醇组分对 α-糖苷酶的抑制效果最强。同时应用 LC-MS 定性分析组 分,发现鹰嘴豆水提物中可能存在鹰嘴豆芽素 A、大豆皂苷 Bb、芒柄花素等物质。说明这些皂苷及异黄酮类化合物影 响 α-葡萄糖苷酶抑制效果。
中文关键词: 鹰嘴豆  α-葡萄糖苷酶  降糖
Abstract:This paper reports the α -glucosidase inhibition of the aqueous extract from different chickpea components is determined. It is found that its half-inhibitory concentration (IC50) to the enzymic activity of α - glycosidase is 0.20 mg/mL. The chickpea aqueous extracts are screened by macroporous resin D101 for evaluation of activity of the different components in it. The results show that through the elution by ethanol of different concentrations, chickpeas aqueous extract shows stronger inhibition effect on α-glycosidase gradually, components with the 40 % ethanol exhibits the strongest inhibitory effect. At the same time, it is determined by LC-MS chromatograms that the different components in the chickpeas aqueous extract may consist of the Biochanin A、Soyasaponin Bb、Formononetin etc, which illustrate that these saponin and isoflavone compounds influence the inhibition effect from α-glycosidase.
文章编号:201605002     中图分类号:    文献标志码:A
基金项目:江苏省普通高校研究生科研创新计划项目(KYLX_1348);江苏省科技支撑项目(BE2011383);江苏省高校自然科学研究重大项目 (12KJA550003)
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