Abstract:The determination of acesulfame potassium，benzoic acid，sorbic acid，saccharin sodium and dehydroacetic in beverage by high performance liquid chromatography（HPLC）. The analysis was performed on a ZORBAX SB-C18 column（4.6 mm×250 mm，5 μm）using methanol and 0.02 mol/L ammonium acetate solution（pH= 6.5，5∶95（Volume ratio）as the elution，with the external standard curve. The linear range of menthod was in the range of 1 μg/mL-200 μg/mL：acesulfame potassium（r=0.999 6），benzoic acid（r=0.999 5），sorbic acid（r=0.999 7），saccharin sodium（r=0.999 5）and dehydroacetic（r=0.999 8）.The recoveries for samples ranged from 95 %-105 %。This method was proved to be simple，rapid and accurate.