本文已被:浏览 363次 下载 0次
投稿时间:2014-11-06
投稿时间:2014-11-06
中文摘要: 建立保健食品中β-胡萝卜素含量的测定方法。采用高效液相色谱法,色谱柱:岛津Inertsil C18(4.0 mm× 75 mm,3 μm),以甲醇∶乙腈=90∶10为流动相,流速1.0 mL/min。结果:β-胡萝卜素的浓度与峰面积线性关系良好(R2=0.999 81),平均回收率为99.4 %,RSD=0.4 %(n=9)。因此,高效液相色谱法可靠、灵敏、快速,可用于保健食品中β-胡萝卜素含量测定。
Abstract:To establish a method for determination of β- carotene in health foods. Methods:high performance liquid chromatography(HPLC),column:using Shimadzu Inertsil C18column(4.0 mm×75 mm,3 μm),Methanol∶Acetonitrile = 90∶10 as the mobile phase,flow rate:1.0 mL/min. The linear relationship between βcarotene concentration and peak area are good(R2= 0.999 81),the average recovery was 99.3 %,RSD = 0.4 % (n = 9).Conclusion:The method is reliable,sensitive,rapid and can be used for determination of β- carotene in health foods.
keywords: β- carotene health food HPLC determination
文章编号:201604038 中图分类号: 文献标志码:
基金项目:
Author Name | Affiliation |
CAI Wei-jiang,HUANG Kang-hui | By-Health Co.,Ltd.,Zhuhai 519040,Guangdong,China |
引用文本: