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食品研究与开发:2016,37(4):-
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高效液相色谱法测定保健食品中β-胡萝卜素
(汤臣倍健股份有限公司,广东珠海519040)
Determination of β- Carotene in Health Foods by HPLC
(By-Health Co.,Ltd.,Zhuhai 519040,Guangdong,China)
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投稿时间:2014-11-06    
中文摘要: 建立保健食品中β-胡萝卜素含量的测定方法。采用高效液相色谱法,色谱柱:岛津Inertsil C18(4.0 mm× 75 mm,3 μm),以甲醇∶乙腈=90∶10为流动相,流速1.0 mL/min。结果:β-胡萝卜素的浓度与峰面积线性关系良好(R2=0.999 81),平均回收率为99.4 %,RSD=0.4 %(n=9)。因此,高效液相色谱法可靠、灵敏、快速,可用于保健食品中β-胡萝卜素含量测定。
Abstract:To establish a method for determination of β- carotene in health foods. Methods:high performance liquid chromatography(HPLC),column:using Shimadzu Inertsil C18column(4.0 mm×75 mm,3 μm),Methanol∶Acetonitrile = 90∶10 as the mobile phase,flow rate:1.0 mL/min. The linear relationship between βcarotene concentration and peak area are good(R2= 0.999 81),the average recovery was 99.3 %,RSD = 0.4 % (n = 9).Conclusion:The method is reliable,sensitive,rapid and can be used for determination of β- carotene in health foods.
文章编号:201604038     中图分类号:    文献标志码:
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