Abstract:Technological conditions of extraction of mulberry leaves with ultrasound-assisted extraction were optimized for developing and utilizing the resource of mulberry leaves， by response surface methodology as the method of studying polysaccharide of mulberry leaves. According to the principle of Box -Behnken central composite design， ultrasonic time， extraction temperature and solid -liquid ratio were chosen as response factors， extraction of Polysaccharide from mulberry leaves （MLP） mass as response value， and a three-factor and three levels central composite design was adopted to determine the influence of various technological conditions. The results showed that better extraction conditions were 52 ℃ of temperature， 36 min of period，40 ∶ 1 （ mL/g） of liquid - solid ratio. Under these conditions， the yield of MLP was 3.21 %.